Comparative Proteomic Analysis of Mature and Immature Oocytes of the Swamp Buffalo (Bubalus bubalis)

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Comparative Proteomic Analysis of Mature and Immature Oocytes of the Swamp Buffalo (Bubalus bubalis)

Maternal protein components change markedly during mammalian oogenesis. Many of these proteins have yet to be characterized and verified. In this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the Swamp Buffalo (Bubalus bubalis). Proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and m...

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15 صفحه اول

Ovaries from sexually mature buffaloes (Bubalus bubalis) were collected irrespective of age, INCIDENCE OF PARTHENOGENETIC DEVELOPMENT OF BUFFALO (BUBALUS BUBALIS) OOCYTES

The incidence of parthenogenetic development of oocytes in routine IVF practices i.e. without any chemical stimuli, was assessed. After 24 h of maturation while washing and transferring to fertilization medium nine (1.39%) oocytes out of 646 oocytes were found cleaved. All those parthenotes (cleaved oocytes) were transferred to embryo culture medium instead of fertilization medium for observing...

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ژورنال

عنوان ژورنال: International Journal of Molecular Sciences

سال: 2016

ISSN: 1422-0067

DOI: 10.3390/ijms17010094